Molecular Biology Scientist- Functional Genomics Job at Insight Global, Cambridge, MA

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  • Insight Global
  • Cambridge, MA

Job Description

We are seeking a scientist to join our Systems Immunology Lab within Inflammation & Immunology Research Unit for a 6 month contract, with likely extensions. The successful candidate will be responsible for generating massive single-cell perturbational data using functional genomics and single cell transcriptomics technologies in collaboration with a small team. You will generate custom lentiviral libraries and maintain extensive cell cultures. Generate edited pools of cells via lentiviral transduction and arrayed electroporation. Generate single-cell data via several commercial workflows, including from 10x Genomics. Develop, modify, and customize such procedures as needed to succeed in specific cell lines and types, and at large scale.

Requirements:

  • Bachelors or Masters + 3 years of experience in Biology, Immunology or a related field
  • 3+ years of hands-on experience with standard cell culture techniques including primary immune cells handling and isolation, maintenance of cell lines and in vitro stimulation of primary immune cells
  • Experience with standard molecular biology techniques including lentivirus infection, qPCR, western blot, and flow cytometry.
  • Previous experience working with Diverse Cell Types
  • Experience with Molecular biology techniques *qPCR
  • Experience in Gene Editing

Plusses:

  • Direct experience in pooled cell screening of immune cells using CRISPRi, CRISPRa, and other methods of in vitro gene editing

Job Tags

Contract work,

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